Role of Caenorhabditis elegans protein phosphatase type 1, CeGLC-7β, in metaphase to anaphase transition during embryonic development

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著者最終稿を登録可能.In Caenorhabditis elegans embryogenesis, phosphorylation events are critical to chromosomal changes. To investigate the dephosphorylation of chromosome behavior, we cloned and characterized the cDNA that encodes C. elegans protein phosphatase type 1 (CeGLC-7β), which is composed of 333 amino acids. CeGLC-7β possesses a highly conserved amino acid sequence with mammalian and Drosophila protein phosphatase 1. Here, we report on the contribution of CeGLC-7β to the dephosphorylation of histone H3 at anaphase. At the embryonic stage, CeGLC-7β is associated with the nuclear membrane and chromosomes. The deletion of the Ceglc-7β gene and a microinjection of double-stranded RNA produce a disorganized embryogenesis. The Ceglc-7β gene mutation causes an abnormal accumulation of phosphorylated histone H3 and delays the mitotic process after anaphase. We propose that CeGLC-7β is involved in chromosome dynamics including histone H3 dephosphorylation. © 2003 Elsevier Science (USA). All rights reserved.
2003-07-15