Expression, localization, and binding activity of the ezrin/radixin/moesin proteins in the mouse testis.
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発行後6ヶ月より最終稿を登録可能.The ezrin, radixin, and moesin (ERM) proteins represent a family of adaptor proteins linking transmembrane proteins to the cytoskeleton. The seminiferous epithelium undergoes extensive changes in cellular composition, location, and shape, implicating roles of the membrane-cytoskeleton interaction. It remains unknown, however, whether the ERM proteins are expressed and play significant roles in the testis. In the present study, we examined the spatiotemporal expression of ERM proteins in the mouse testis by Western blotting and immunohistochemistry. Ezrin immunoreactivity was demonstrated in the cytoplasm of steps 15 and 16 spermatids from 5 weeks postpartum through adulthood, whereas radixin immunoreactivity was in the apical cytoplasm of Sertoli cells from 1 week through 2 weeks postpartum. No immunoreactivity for moesin was detected at any age. Immunoprecipitation demonstrated that ezrin was bound to the cytoskeletal component actin, whereas radixin was bound to both actin and tubulin. Of the transmembrane proteins known to interact with ERM proteins, only cystic fibrosis transmembrane conductance regulator, a chloride transporter, was bound to ezrin in elongated spermatids. These results suggest that ezrin is involved in spermiogenesis whereas radixin is involved in the maturation of Sertoli cells, through interaction with different sets of membrane proteins and cytoskeletal components.
論文 | ランダム
- 薄層クロマトグラフィーの塗料分野への利用 (色材のcharacterization最近の機器分析の応用(特集))
- 最近の橋カケ硬化形塗料用樹脂
- リノール酸変性アルキド樹脂合成過程における無水マレイン酸挙動について
- アルキド樹脂中の遊離脂肪酸について
- 総論:紫外線照射による塗膜の硬化 (色材工業における感光性樹脂(テーマ資料))