Microbial resolution of Nε-acetyl-DL-lysine with Rhodococcus sp. AIU Z-35-1

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An enantioselective resolution of Nε-acetyl-dl-lysine was investigated using cells from Rhodococcus sp. AIU Z-35-1 cultivated with l-lysine medium. Nε-Acetyl-l-lysine was deaminated by the resting cells, and 6-acetylamino-2-oxohexanoic acid was formed, but Nε-acetyl-d-lysine was not. This reaction was optimum at pH 6.5 and 30 °C, and the highest reaction speed was obtained by cells harvested after 1 day of cultivation. When 150 mM Nε-acetyl-dl-lysine was incubated at pH 6.5 and 30 °C for 5 days with cells harvested after 1 day of cultivation, more than 98% of the Nε-acetyl-l-lysine was converted to 6-acetylamino-2-oxohexanoic acid, and Nε-acetyl-d-lysine completely remained. Thus, Nε-acetyl-d-lysine was enantioselectively produced from Nε-acetyl-dl-lysine by the cell reaction with Rhodococcus sp. AIU Z-35-1. This cell reaction was also useful for the efficient production of 6-acetylamino-2-oxohexanoic acid from Nε-acetyl-l-lysine.http://www.elsevier.com/wps/find/journaldescription.cws_home/525211/description#description