Epithelialization in Oral Mucous Wound Healing in Terms of Energy Metabolism
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概要
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Purpose: The purpose of this study was to clarify the "healing capacity" of woundsof the oral mucosa in comparison to those of the skin, and to evaluate the woundhealing mechanism of oral mucosa using a cytobiological approach from the aspect ofenergy metabolism in oral keratinocytes.Materials and methods: Samples of epidermal and oral keratinocytes collected atsurgery and of cultured oral keratinocytes were used to analyze (1) by gaschromatography the composition of fatty acids (16:0, 18:2, 20:4) in the cell membranesof keratinocytes, (2) by immunohistochemical staining of GLUT-1 antibody and specificPAS staining the localization of glucose metabolism, and (3) by RT-PCR and Westernblotting the expression of GLUT-1 mRNA and of protein in the keratinocytes of thebasal and parabasal layers of each epithelial tissue.Results: 1. The % composition of palmitic acid (16:0) was significantly higher inbuccal mucosal keratinocytes (27.18±3.74%) and in the gingiva (23.00±1.40%) than inthe epidermis (17.54±0.37%). 2. Immunohistochemical staining showed GLUT-1protein in the skin to be expressed only in the bulge region of hair follicles and in theepidermal basal layer, and observed nearly throughout all epithelial cell layers in theoral mucosa. 3. PAS-positive cells were observed among differentiation-enhanced cellsin the upper prickle layer in the oral mucosa. 4. The same results were obtained fromRT-PCR and a Western blotting analysis.Conclusion: The present study demonstrated definite cytobiological evidence that the oral mucosa surpasses the skin in regard to its wound healing capacity.
- 神戸大学医学部の論文
- 2009-02-00
神戸大学医学部 | 論文
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