Immunohistochemical localisation of d-β-aspartic acid-containing proteins in climatic droplet keratopathy
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概要
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Aim: Biologically uncommon D-β-aspartic acid (D-β-Asp) accumulates in the body with age and is involved in the ageing process. In the present study, the localisation of D-β-Asp-containing proteins was investigated in surgical specimens with climatic droplet keratopathy (CDK), one of the ocular changes related to the ageing process.Methods: Immunohistochemical localisation of D-β-Asp-containing proteins using polyclonal antibodies raised against D-β-Asp-containing peptides was examined in three corneas with CDK, three corneas with interstitial keratitis, six corneas with bullous keratopathy, and three corneas without any corneal diseases.Results: Strong immunoreactivity to D-β-Asp-containing peptide was detected in all the surgical specimens with CDK. In contrast, no immunoreactivities to D-β-Asp-containing peptides were detected in the surgical specimens with bullous keratopathy, interstitial keratitis, or no corneal diseases.
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