DNA microarray analysis of redox-responsive genes in the genome of the cyanobacterium Synechocystis sp strain PCC 6803

概要

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The whole genome DNA microarrays were used to evaluate the effect of redox state of photosynthetic electron transport chain on gene expression of Synechocystis sp. PCC 6803. Two specific inhibitors of electron transport, 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) or 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB), were added to the cultures and changes in accumulation of transcripts were examined. About 140 genes were highlighted as reproducibly affected by the change of redox state of photosynthetic electron transport chain. It was shown that some stress-responsive genes but not photosynthetic genes were under the control of the redox state of the plastoquinone pool in Synechocystis sp. PCC 6803.