The roles of the quorum-sensing system in the release of extracellular DNA, lipopolysaccharide, and membrane vesicles from Pseudomonas aeruginosa.
スポンサーリンク
概要
- 論文の詳細を見る
Biofilms play an important role in the establishment of chronic infection caused by Pseudomonas aeruginosa. It has been suggested that membrane vesicles (MVs) are released into the surrounding medium during normal growth and might supply the bacterial extracellular DNA that is required for early biofilm formation, as MVs released from the bacterial outer membrane are suspected to be the source of extracellular DNA. MVs possess lipopolysaccharide (LPS), extracellular DNA, and several hydrolytic enzymes. It is well known that the quorum-sensing (QS) system is important in controlling virulence factors in P. aeruginosa and biofilm formation. In the current study, we investigated extracellular LPS and DNA in the supernatants of culture solutions from PAO1, the wild-type P. aeruginosa, and those of QS mutants. As compared to that of las QS mutants, the amount of LPS and DNA released was significantly higher in PAO1 and in las QS mutants complemented with N-(3-oxododecanoyl) homoserine lactone. Our study indicated that the QS is among the regulators involved in the release of extracellular DNA and LPS. It is possible that these extracellular components are supplied from MVs. Investigation of the mechanism of biofilm formation is of particular interest, as it may be useful for designing treatments for severe P. aeruginosa infection.
- National Institute of Infectious Diseasesの論文
著者
-
Hirakata Yoichi
長崎大学 医学部第二内科学
-
Izumikawa Koichi
長崎大学 医学部第二内科
-
Izumikawa Koichi
北九州市立八幡病院
-
IZUMIKAWA Kohichi
the Department of Laboratory Midicine
-
Nakayama Seiko
Dep. Of General Medicine Nagasaki Univ. School Of Medicine
-
Izumikawa K
長崎大学 医学部第二内科
-
Hirakata Y
長崎大学 医学部第二内科学
-
Higami Yoshikazu
Department Of Histology And Cell Biology Nagasaki University Graduate School Of Biomedical Sciences
-
Hirakata Yoichi
Department Of Clinical Laboratory Medicine Nagasaki University School Of Medicine
-
Harada Yousuke
Department Of Laboratory Medicine Nagasaki University Graduate School Of Biomedical Sciences
-
Hara Yukiko
Department Of Laboratory Medicine Nagasaki University Graduate School Of Biomedical Sciences
-
Hirakata Yoichi
Department Of Clinical Microbiology And Management And Analysis Of Infectious Diseases Tohoku University Graduate School Of Medicine
関連論文
- Low Expression of T-cell Co-stimulatory Molecules in Bone Marrow-Derived Dendritic Cells in a Mouse Model of Chronic Respiratory Infection with Pseudomonas Aeruginosa
- Two Patients Who Developed Leptospirosis-Associated Acute Renal Failure within the Same Season
- Antimicrobial susceptibility of respiratory tract pathogens in Japan during PROTEKT years 1-3 (1999-2002)
- Measurement of (1-3)-β-D-Glucan Derived From Different Gauze Types
- Two sporadic cases of infections due to Klebsiella pneumoniae resistant to expanded-spectrum cephalosporins in Japan
- Analysis of Genetic Relationships and Antimicrobial Susceptibility of Verotoxin-Producing Escherichia coli Strains Isolated in Nagasaki Prefecture, Japan in 1996
- In vivo Retrovirus-mediated Herpes Simplex Virus Thymidine Kinase Gene Therapy Approach for Adult T Cell Leukemia in a Rat Model
- Serum Levels of Soluble Fas/APO-1 Receptor in Human Retroviral Infection and Associated Diseases
- The Fas/Fas-ligand system functions in hepatocytes in the early stage of fulminant hepatic failure in rats
- Comparison of Complement Fixation and Microimmunofluorescence Tests in Respiratory Infections Caused by Chlamydia and an Evaluation of the Serum Amyloid A Protein in Chlamydial Infections
- Effectiveness of Intravenous Minocycline in Patients with Mycoplasmal Pneumonia
- Microbiological Studies of Four Patients Extensively burned by the Pyroclastic Flow of Mt. Fugen in Unzen, Japan
- Potential Activity of Carrageenan to Enhance Antibacterial Host-Defense Systems in Mice
- Lung fibrosis 10 years after cessation of bleomycin therapy.
- A Probable Case of Aspiration Pneumonia Caused by Candida glabrata in a Non-neutropenic Patient with Candidemia
- Clinical Characteristics of Pneumonia Caused by Penicillin Resistant and Sensitive Streptococcus pneumoniae in Japan
- Antifungal activity of a new triazole, voriconazole(UK-109496), against clinical isolates of Aspergillus spp
- Antimicrobial Activity of Saturated Fatty Acids and Fatty Amines against Methicillin-Resistant Staphylococcus aureus
- Lemierre's syndrome followed by acute respiratory distress syndrome successfully rescued by antibiotics and hemoperfusion with polymyxin B-immobilized fiber.
- Elevated Levels of Tenascin-C in Patients with Cryptogenic Organizing Pneumonia
- The roles of the quorum-sensing system in the release of extracellular DNA, lipopolysaccharide, and membrane vesicles from Pseudomonas aeruginosa.
- Sarcoidosis Accompanied by Systemic Lupus Erythematosus and Autoimmune Hepatitis
- A case of Legionella pneumophila pneumonia followed by invasive aspergillosis.
- Adult Still's disease associated with cytomegalovirus infection
- A case of splenic abscess during treatment of interstitial pneumonia.
- Adhesion-dependent growth of primary adult T cell leukemia cells with down-regulation of HTLV-I p40Tax protein : a novel in vitro model of the growth of acute ATL cells
- Possible Attenuation of Fas-Mediated Signaling by Dominant Expression of Caspase-8 Aberrant Isoform in Adult T-Cell Leukemia Cells
- Clinical Relevance of Survivin as a Biomarker in Neoplasms, Especially in Adult T-Cell Leukemias and Acute Leukemias
- Detection of Mycobacterium tuberculosis in Preserved Tuberculous Lymph Nodes by Polymerase Chain Reaction
- Role of Motility in the Endogenous Pseudomonas aeruginosa Sepsis after Burn
- Bacterial Translocation of Intestinal Pseudomonas aeruginosa in Post-Burn Infection of Mice
- Two Coincidental Isolated Cases of Legionnaire's Disease Diagnosed by PCR and Cured by Combination Therapy with Clarithromycin and Rifampicin
- High-risk populations for nasal carriage of methicillin-resistant Staphylococcus aureus
- Review Article Invasiveness of Pseudomonas aeruginosa and Its Role in Diversity of Pseudomonal Infectious Diseases
- The first telithromycin-resistant Streptococcus pneumoniae isolate in Japan associated with erm(B) and mutations in 23S rRNA and riboprotein L4.
- Quorum Sensing System Lactones Do Not Increase Invasiveness of a MexAB-OprM Efflux Mutant but Do Play a Partial Role in Pseudomonas aeruginosa Invasion
- Spontaneous rupture of non-aneurysmal ascending aorta
- Minigemistocytic astrocytoma with frequent apoptoses : Analysis of tumor growth
- Evaluation of Newly Developed Oxygen Meters with Multi-Channels and Disposable Oxygen Electrode Sensors for Antimicrobial Susceptibility Testing
- Low Expression of T-cell Co-stimulatory Molecules in Bone Marrow-Derived Dendritic Cells in a Mouse Model of Chronic Respiratory Infection with Pseudomonas Aeruginosa
- Monolayer Culture Systems with Respiratory Epithelial Cells for Evaluation of Bacterial Invasiveness
- Disease Severity in Patients with Simultaneous Influenza and Bacterial Pneumonia
- Development of interpretive criteria for tebipenem disk diffusion susceptibility testing with Staphylococcus spp. and Haemophilus influenzae
- Methicillin resistant Staphylococcus aureus in a Japanese community hospital : 5-year experience
- Clinical Features of Bacteremia Caused by Methicillin-Resistant Staphylococcus aureus in a Tertiary Hospital
- Clinical Characteristics of Seven Patients with Aeromonas Septicemia in a Japanese Hospital
- Characteristics and disease severity of healthcare-associated pneumonia among patients in a hospital in Kitakyushu, Japan
- Altered lipid metabolism in rodents subjected to calorie restriction
- Active surveillance of methicillin-resistant Staphylococcus aureus with the BD GeneOhm MRSA™ assay in a respiratory ward in Nagasaki, Japan
- Immediate Single Lobar Retransplantation for Primary Graft Dysfunction After Living-Donor Lobar Lung Transplantation : Report of a Case
- A report from the committee on microbroth dilution antimicrobial susceptibility testing : Issues in antimicrobial susceptibility testing of Haemophilus influenzae : a comparison of the Japanese Society of Chemotherapy lysed horse blood and the CLSI HTM br
- A new ketolide antibiotic, telithromycin and its effects on Mycoplasma pneumoniae
- The efficacy and safety of high-dose arbekacin sulfate therapy (once-daily treatment) in patients with MRSA infection
- Clinical analysis of fulminant Mycoplasma pneumoniae pneumonia with hypoxia
- Bactericidal effects of antimicrobial agents on epithelial cell-associated Pseudomonas aeruginosa
- Possible Pulmonary Cryptococcosis in a Patient with Crohn's Disease during Anti-Tumor Necrosis Factor-Alpha Treatment: a Case Report and Literature Review
- In vivo efficacy and pharmacokinetics of biapenem in a murine model of ventilator-associated pneumonia with Pseudomonas aeruginosa