Deficiency of ribosomal protein S19 during early embryogenesis leads to reduction of erythrocytes in a zebrafish model of Diamond-Blackfan anemia
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概要
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Ribosomes are responsible for protein synthesis in all cells. Ribosomal protein S19 (RPS19) is one of the 79 ribosomal proteins (RPs) in vertebrates. Heterozygous mutations in RPS19 have been identified in 25% of patients with Diamond-Blackfan anemia (DBA), but the relationship between RPS19 mutations and the pure red-cell aplasia of DBA is unclear. In this study, we developed an RPS19-deficient zebrafish by knocking down rps19 using a Morpholino antisense oligo. The RPS19-deficient animals showed a dramatic decrease in blood cells as well as deformities in the head and tail regions at early developmental stages. These phenotypes were rescued by injection of zebrafish rps19 mRNA, but not by injection of rps19 mRNAs with mutations that have been identified in DBA patients. Our results indicate that rps19 is essential for hematopoietic differentiation during early embryogenesis. The effects were specific to rps19, but knocking down the genes for three other RPs, rpl35, rpl35a and rplp2, produced similar phenotypes, suggesting that these genes might have a common function in zebrafish erythropoiesis. The RPS19-deficient zebrafish will provide a valuable tool for investigating the molecular mechanisms of DBA development in humans.
論文 | ランダム
- 2S2-S5 高校・大学・その先の未来へ : 理系キャリア形成支援に必要な科学技術コミュニケーション(理系キャリアデザインと科学コミュニケーション(一般公開),CoSTEP企画シンポジウム,転換期の科学教育)
- 参加型社会における科学技術コミュニケーション(21世紀の科学技術社会を推進する高等科学教育の課題-サイエンス・コミュニケーターをいかに養成すべきか-)
- 信号の伝送技術 (最近の計測システム(特集))
- ひろばのすくすく子育てアドバイス(12)先生と仲良くなりたいな
- 遠隔監視制御方式・装置の現状 (最近の遠隔測定制御システム(特集))