Insulin-stimulated Fusion of GLUT4 Vesicles to Plasma Membrane is Dependent on Wortmannin-sensitive Insulin Signaling Pathway in 3T3-L1 Adipocytes

概要

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It is established that wortmannin which completely inhibits class IA PI 3-kinaseactivation abrogated the insulin-dependent translocation of GLUT4 to the plasmamembrane in adipocytes and skeletal muscle. However, it was not clear which stepswortmannin inhibited during the whole translocation process of GLUT4. We have nowdissected the each steps of the GLUT4 trafficking in 3T3-L1 adipocytes usingexogenously-expressed GLUT4 reporter in combination with plasma membrane lawnassay. We showed that 100 nM wortmannin inhibited the fusion of GLUT4 vesicles tothe plasma membrane without affecting the movement and the subsequenttethering/docking event of GLUT4 vesicles to the membrane in 3T3-L1 adipocytes.These results suggest that wortmannin-sensitive insulin signaling pathway plays acrucial role in the fusion step of GLUT4 vesicles to the plasma membrane in 3T3-L1adipocytes.
神戸大学医学部の論文
2008-08-00