墨汁法による細菌のGram反応鑑別に関する知見補遺

元データ 1952-06-28 千葉医学会 著者(集計中)

概要

The results obtained in the experiments described in this paper were as follows. 1. The characteristic feature of the Gram-negative intestinal bacteria, in the India-ink smear, which had been first reported by P. Eisenberg (1910), lies in the fact, that each of organisms showed a round or elliposoid dark spot in central part of the cell. The same may be observed on Escherichia coli, Aerobacter aerogenes, Eberthella typhosa, Salmonella enteritidis, Shigella Dysenteriae, Shigella Flexneri 2 a (Komagome B-III), Proteus OX-19, and Neisseria gonorrhoeae. 2. With the exception of Diplococcus pneumoniae (type III), Staphylococcus aureus, Streptococcus haemolyticus, Bacillus subtilis, and Corynebacterium diphtheriae showed no central dark spot in their cells; on the contrary, the India-ink particles were distributed to the direct surroundings of each organism forming dark halos. The intensity of halo-formation seemed to be related with the degree of Gram-positiveness of each species, and was most distinct with Staph. aureus and B. subtilis, and least marked with Coryn. diphtheriae, and Str. haemolyticus and Diploc. pneumoniae etc. lying between both extremities. 3. A strain of B. subtilis (NRRL 558) has spontaneously transformed itself into Gram-negative state in the course of successive transplantation on agar plants, and at the same time, its characteristic behavior against India-ink turned gradually into that of Gram-negative ones. 4. Gram-positive state of organisms was easily lost by acid or alkali treatment, but the original characteristics in the India-ink smear were not easily transformed into that of Gram-negative organisms. 5. With one exception of Staph. aureus, Gram-positive nature was lost by stripping with bile salts as well as with ribonuclease. This treatment, however, could not change the characteristic figures in the India-ink smear. 6. According to Henry and Staccy (1934), it was possible to restore the lost Gram-positive state of B. subtilis resulting by extraction with bile salts by placing the washe

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