In Vitro Plantlet Regeneration of Paraserianthes falcataria (L..) Nielsen
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Plantlets were regenerated in vitro from seedling tissue of Paraserianthes falcataria (L.) Nielsen, which is a fast-growing, tropical tree of the Leguminosae family. Axillary buds and cotyledonary nodes of seedlings that were germinated under aseptic conditions were used as explants. Various concentrations of BAP were added to three kinds of basic media, WPM, B5 and MS, in order to find a suitable medium for shoot proliferation. Shoot induction was seen in all combinations of culture medium. When axillary buds were used as explants, the greatest number of shoots developed (4.2 per explant) when the explants were cultured on B5 medium with 0.22mg/l BAP. When cotyledonary nodes were used as explants, many shoots were induced (5.4 to 5.7 per explant) on B5 medium with 0.22mg/l to 0.44mg/l of BAP. Although shoot elongation in axillary buds and cotyledonary nodes was most pronounced on hormone-free medium, few shoots per explant were induced. When shoots elongated to 0.5cm or more were cultivated on a hormone-free MS medium, 90% of the shoots rooted. All plantlets cultivated for six weeks on rooting medium survived transplantation and acclimatization to vermiculite-filled pots.熱帯産マメ科早生樹であるParaserianthes falcataria (L.) Nielsenの芽生えの組織から，試験管内で植物体を再生させた。外植体として，無菌発芽させた芽生えの腋芽と子葉節を用いた。WPM，B5，MSの3種類の基本培地にBAPを様々な濃度で添加し，シュートの増殖に適した培地を検索した。すべての組み合わせの培地でシュートの発生が見られた。腋芽を外植体とした場合は，B5培地にBAPを0.22mg/l添加した培地でもっとも多くのシュート（外植体1つ当たり4.2本）が見られた。子葉節を外植体とした場合には，B5培地にBAPを0.22mg/lから0.44mg/l添加した培地で，多くのシュート（外植体1つ当たり5.4本から5.7本）ができた。外植体が腋芽と子葉節のどちらの時にも，3種類の基本培地すべてで，ホルモンフリーの時にシュートの成長がもっともよかったが，外植体当たりのシュートの数は少なかった。0.5cm以上に伸長したシュートをホルモンフリーのMS培地で培養したところ，90％のシュートが発根した。バーミキュライトを入れたポットに発根培地で6週間培養した幼植物体を移植し順化したところ，すべての植物体が生存した。
- 東京大学大学院農学生命科学研究科附属演習林,The Tokyo University Forests,Laboratory of Tissue Culture, Faculty of Forestry, Mulawarman University,Arboricultural Research Institute, University Forests, Graduate School of Agricultural and Life Sciences, The University of Tokyo,Lの論文