A continuous-flow ATP amplification system for increasing the sensitivity of quantitative bioluminescence assay
スポンサーリンク
概要
- 論文の詳細を見る
We constructed a novel ATP amplification reactor using a continuous-flow system, and this allowed us to increase the sensitivity of quantitative bioluminescence assay by controlling the number of ATP amplification cycles. We previously developed a bioluminescence assay coupled with ATP amplification using a batch system. However, it was difficult to control the number of amplification cycles. In this study, ATP amplification was performed using a continuous-flow system, and significant linear correlations between amplified luminescence and initial ATP concentration were observed. When performing 4 cycles of continuous-flow ATP amplification, the gradient of amplification was 1.87N. Whereas lower quantifiable level was 500 pM without amplification, values as low as 50 pM ATP could be measured after amplification. Sensitivity thus increased 10-fold, with further improvements expected with additional amplification cycles. The continuous-flow system thus effectively increased the sensitivity of quantitative bioluminescence assay.
- 2008-08-01
論文 | ランダム
- 7. 地域に於けるコミュニケーションの場について1 : 農村地域(舟引町美山地区)の場合(その1)(歴史,建築計画)
- 4. 地域に於けるコミュニケーションの場について2 : 農村地域(舟山町美山地区)の場合(その2)(歴史,建築計画)
- 141 ベンゼン・イソプロピルアルコール取り扱い作業場の環境測定と作業者の個人曝露量、尿中代謝物について(有機溶剤,一般講演,第9回日本産業衛生学会・第40回日本産業医協議会講演記録)
- 5.鉛中毒認定者の尿中コプロポルフリン,δアミノレブリン酸,血中鉛量の変動(第25回近畿産業衛生学会)
- 209. ベンゼン,フェノール取扱い作業者の尿中フェノール量について(有機溶剤,一般講演,第58回日本産業衛生学会・第38回日本産業医協議会)