Calcitonin gene-related peptide increases K-ras expression in primary culture cell of rat skeletal muscle

概要

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Calcitonin gene-related peptide (CGRP) coexists with acetylcholine in the motor nerve terminal and may act as nerve-derived trophic factor for muscle. In this report, we show that CGRP increases intracellular cAMP as a second messenger and induces K-ras expression, which is one of the proto-oncogenes regulating cell function. In cultured rat myotube, CGRP increased the intracellular cAMP level but did not change the cGMP content. The expressions of proto-oncogenes in cultured rat myotube were measured by Northern blot analysis. The expressions of erbA, src, raf, sis, fos, myc, H-ras and K-ras were detected, but erbB and myb were not seen. CGRP increased solely K-ras expression accompanied with the increased expression of nicotinic acetylcholine receptor (AChR) α-subunit. These findings suggest that CGRP is a nerve-derived trophic factor controlling gene expressions in the skeletal muscle.
BioMed Centralの論文
1996-00-00