無支柱栽培トマトの加工適性に関する研究-4-トマト果実のカロチノイド定量法の検討
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Color is the most important factor in tomato products. Therefore, some convenient methods of carotenoid determination in tomato fruits or products are necessary to be presented. Three methods for carotenoid determination have generally been used such as below. a. The method by using pure carotenoids seperated from tomato fruits at each experiment for standard pigments. b. The method by using azobenzene for standard pigments. c. The method by using the absorbance calculated from the extinction coefficient previously reported. In the present study, the absorption spectra of carotenoid pigments obtained by above methods were compared with one another and tried to find more convenient method for determination of carotenoids. The results obtained were shown as follows. 1. We found a pretty discrepancy among the absorbances of β-carotene at the wave length of 448nm by these three methods. On the contrary, we found a considerable contiguity among those at 430nm. 2. We found also a pretty discrepancy among the absorbances of lycopene at 470nm, however, we found a considerable contiguity among those at 480nm. 3. From these results, when azobenzene is used as standard pigment to make the calibration curve, the wave length for measurement or the coefficient for calculation requires further examination. 4. The confirmation of pure carotenoid pigments seperated from tomato fruits has been thought to be difficult. The method by utilization of azobenzene as a standard pigment, therefore, is surely a good one, but we dare say that the best method is to make use of the extinction coefficients established,
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