リンドウ属花粉の発芽および貯蔵に関する研究
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概要
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Germination and storage condition of Gentiana pollen were studied. Sucrose solution was used as culture medium. The optimum sucrose concentration of medium was 10% in G. triflora and G. Zollingeri, and 15% in G. scabra var. Buergeri. However, percent germination of them was not so varied in wide range of sucrose concentration. Boric acid of 100 mg/l in medium stimulated germination of G. scabra var. Buergeri pollen, especially in the pollen of low viability. The optimum temperature for pollen germination was 25℃ both in G. triflora and in G. scabra var. Buergeri. Pollen germination increased after anthesis in G. triflora and G. scabra var, Buergeri. On the day of anthesis, percent germination of pollen was not so markedly varied by time. Pollen of G. triflora was enclosed in capped small vials with silica-gel and stored at -15℃, 2～5℃ and room temperature. Longevity of the pollen increased in dry and low (2～5℃ or -15℃) temperature condition, and decreased in high relative humidity and room temperature condition. The pollen of Ga triflora stored at 2～5℃ dry condition showed germination of 6% even after 454 days. In G. scabra var. Buergeri pollen stored as same condition as G. triflora, 38% germination was observed after 360 days, and germination was observed in several individuals even after 547 days of storage. The pollen of G. Zollingeri, G. septemfida, G. tibetica and G. pneumonanthe stored at dry and low temperature condition showed viability over 2 or 3 months. The viability of stored pollen of G. triflora was tested actually by pollination. The pollen stored 425 days produced fruits and seed. By the germination test, it was proved that the seed germinated and produced seedlings. From the results obtained here, dry and low temperature storage is effective for keeping longevity of Gentiana pollen.
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