Cytosolic Ca2+ alteration mediates both ryanodine receptor and IP3 receptor in TE671/RD cells

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TE671 is a cell line obtained from the rhabdomyosarcoma cells. In the present study, we examined [Ca2+]i alteration induced by acetylcholine (ACh) in TE671/RD cells with the special attention to ryanodine receptor (RyR) and inositol 1,4,5-triphosphate receptor (IP3R). The change of [Ca2+]i was mediated by muscarinic type 3 (m3) AChR. Both phosphatidylinositol-specific phospholipase C blocker (U73122) and IP3R blocker (2-APB) inhibited ACh-induced [Ca2+]i elevation, suggesting that IP3 pathway is involved in [Ca2+]i alteration. Ryanodine and FK506 increased ACh-induced [Ca2+]i elevation, which was decreased by RyR blocker (ruthenium red). These results suggest that RyR and FK506 binding protein 12 kDa (FKBP12) complex was involved in [Ca2+]i change, and that TE671/RD cell line has a hybrid characteristic of smooth and skeletal muscles. Copyright © 2005 Biomedical Research Press.