Effect of Inhibition of Proteasome-Mediated Proteolysis on Ligninolytic Activities of White-Rot Fungi
スポンサーリンク
概要
- 論文の詳細を見る
It has recently been established that most short- and long-lived cellular proteins (80-90%) are degraded by a highly selective non-lysosomal pathway that requires ATP and a large (~2.5 MDa) multisubunit, multicatalytic proteinase complex known as the 26S proteasome. It degrades many important proteins involved in signaling pathway, in cell cycle control, and in general metabolism, including transcription factors and key metabolic enzymes. Here, we demonstrated all distinct proteasome activities: chymotrypsin-like, trypsin-like, and caspase-like (peptidylglutamyl-peptide hydrolyzing) in mycelial extracts of the white-rot fungi Trametes versicolor and Phlebia radiata by monitoring cleavage of three different fluorogenic peptide substrates: Suc-LLVY-MCA, Z-GGR-MCA, Z-LLE-βNA, respectively. We also found that this cleavage was ATP-dependent. Reagents that inhibit proteasome-mediated protein degradation in intact cells have recently become available, including substrate-related peptide aldehydes. These inhibitors are useful tools to demonstrate that a process exhibits proteasome-dependent biochemical regulation. In the present study, we report that in vivo Cbz-LLLal treatment strongly inhibited all tested proteasome activities and affected ligninolytic activities in nutrient deprived cultures of both fungi.
- 2008-10-28
論文 | ランダム
- PP-500 Cockcroft-Gaultの推算式によるCcr値と蓄尿法による実測Ccr値は相関するか(発表・討論,第98回日本泌尿器科学会総会)
- PP-394 膀胱腫瘍に対する膀胱全摘、尿路変更術症例の術後合併症の検討(発表・討論,第98回日本泌尿器科学会総会)
- 臨床室 腰椎手術後28年で摘出された遺残ガーゼ肉芽腫の1例
- 非定型抗酸菌感染によって橈尺骨および手根骨全体におよぶ骨髄炎をきたし血管柄付き腓骨移植術を要した1例
- 副腎原発性悪性リンパ腫--6症例の報告と本邦報告例の文献的検討