Effect of Inhibition of Proteasome-Mediated Proteolysis on Ligninolytic Activities of White-Rot Fungi
スポンサーリンク
概要
- 論文の詳細を見る
It has recently been established that most short- and long-lived cellular proteins (80-90%) are degraded by a highly selective non-lysosomal pathway that requires ATP and a large (~2.5 MDa) multisubunit, multicatalytic proteinase complex known as the 26S proteasome. It degrades many important proteins involved in signaling pathway, in cell cycle control, and in general metabolism, including transcription factors and key metabolic enzymes. Here, we demonstrated all distinct proteasome activities: chymotrypsin-like, trypsin-like, and caspase-like (peptidylglutamyl-peptide hydrolyzing) in mycelial extracts of the white-rot fungi Trametes versicolor and Phlebia radiata by monitoring cleavage of three different fluorogenic peptide substrates: Suc-LLVY-MCA, Z-GGR-MCA, Z-LLE-βNA, respectively. We also found that this cleavage was ATP-dependent. Reagents that inhibit proteasome-mediated protein degradation in intact cells have recently become available, including substrate-related peptide aldehydes. These inhibitors are useful tools to demonstrate that a process exhibits proteasome-dependent biochemical regulation. In the present study, we report that in vivo Cbz-LLLal treatment strongly inhibited all tested proteasome activities and affected ligninolytic activities in nutrient deprived cultures of both fungi.
- 2008-10-28
論文 | ランダム
- P-679 肺原発 large cell neuroendocrine carcinoma の2切除例(症例11, 第47回日本肺癌学会総会)
- 動脈性出血を来し緊急手術を要した難治性再燃型小児潰瘍性大腸炎の1例
- 3.胃カンジダ症による胃破裂の1例(第74回日本小児外科学会北海道地方会)
- 14.会陰部腫瘤の3例-Human tailについての考察(第72回日本小児外科学会北海道地方会)
- 鼠径部転移リンパ節で扁平上皮癌への分化を呈した肛門管原発未分化癌の1例