Suppressive Effect of Modified Dominant Negative RAS Mutant on Human Cancer by Gene Transfer with Non-Viral Vector
スポンサーリンク
概要
- 論文の詳細を見る
N116Y, H-RAS mutant, has dominant negative activity in the RAS function and a suppressive effect on the growth of various types of cancer cells. However, a replication error of N116Y is of potential concern for carcinogenesis in clinical application. To decease the concern, we constructed modified N116Y by deleting the carboxyl terminus, which is necessary for the oncogenic function of Ras. One of the C-terminal deletion mutants of N116Y, N116Y-Cdel2 showed a growth-suppressing effect on various human cancer cell lines in vitro: the cervical cancer cell line HeLa, the pancreatic cancer cell line PCI43, the colon cancer cell lines SW480 and LoVo, and the tongue cancer cell line SAS. In addition, the suppressive effect of N116Y-Cdel2 on LoVo cells was also observed in vivo using a non-viral gene transfer vector, HVJ envelope. Our experiments suggest that the modified N116Y is a potential candidate gene for human cancer gene therapy.
- Landes Bioscienceの論文
Landes Bioscience | 論文
- Photosynthesis-dependent Ca2+influx and functional diversity between phospholipases in the formation of cell polarity in migrating cells of red algae
- Effects of cell wall synthesis on cell polarity in the red alga Porphyra yezoensis
- Suppressive Effect of Modified Dominant Negative RAS Mutant on Human Cancer by Gene Transfer with Non-Viral Vector
- Alternative efficacy-predicting markers for paclitaxel instead of CHFR in non-small cell lung cancer
- Protective role of MyD88-independent innate immune responses against prion infection