Up-regulation of P450arom and down-regulation of Dmrt-1 genes in the temperaturedependent sex reversal from genetic males to phenotypic females in a salamander

概要

論文の詳細を見る
The original publication is available at http://www.springerlink.com/When larvae of the salamander Hynobius retardatus were reared at a hightemperature (28°C) during their thermosensitive period (TSP: from 15 to 30 days after hatching),all larvae developed to phenotypic females irrespective of their genetic sexes. Hynobius P450aromatase (P450arom) and Dmrt-1 cDNAs were isolated and their expression pattern wasanalyzed by competitive and conventional RT-PCR. While P450arom gene was expressedpredominantly in the ovary, Dmrt-1 was expressed exclusively in the testis. When larvae werereared at the female-producing temperature (28°C) during the TSP, strong expression of theP450arom and complete suppression of the Dmrt-1 genes were induced in all experimentallarvae. Up-regulation of the P450arom and down-regulation of Dmrt-1 genes even in geneticmales constitute a part of the molecular biological cascade for the temperature-dependent sexreversal from genetic males to phenotypic females in this salamander.