Abundant distribution of TARP γ-8 in synaptic and extrasynaptic surface of hippocampal neurons and its major role in AMPA receptor expression on spines and dendrites
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概要
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Transmembrane AMPA receptor regulatory proteins (TARPs) play pivotal roles in AMP A receptor trafficking and gating. Here we examined cellular and subcellulardistribution of TARP γ-8 in the mouse brain. Immunoblot and immunofluorescence revealed the highest concentration of γ-8 in the hippocampus. Immunogold electron microscopy demonstrated dense distribution of γ-8 on the synaptic and extrasynapticsurface of hippocampal neurons with very low intracellular labeling. Of the neuronalsurface, γ-8 was distributed at the highest level on asymmetrical synapses of pyramidalcells and interneurons, whereas their asymmetrical synapses selectively lackedimmunogold labeling. Then, the role of γ-8 in AMPA receptor expression was pursuedin the hippocampus using mutant mice defective in the γ-8 gene. In the mutant CA1 region, synaptic and extrasynaptic AMPA receptors on dendrites and spines were severely reduced to 35-37% of control levels, whereas reduction was mild for extrasynaptic receptors on somata (74%) and no significant decrease was seen for intracellular receptors within spines. In the mutant CA3 region, synaptic AMPA receptors were reduced mildly atasymmetrical synapses in the stratum radiatum (67% of control level), and showed no significant decrease at mossy fiber-CA3 synapses. Therefore, γ-8 is abundantly distributed on hippocampal excitatory synapses and extrasynaptic membranes, and plays an important role in increasing the number of synaptic and extrasynaptic AMPA receptors on dendrites and spines, particularly, in the CA1 region. Variable degrees of reduction further suggest that other TARPs may also mediate this function at different potencies depending on hippocampal subregions, input sources, and neuronal compartments.
- Blackwell Publishingの論文
著者
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Abe Manabu
Graduate School Of Environmental Studies Nagoya University
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Watanabe Masahiko
Department Of Anatomy School Of Medicine Hokkaido University
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Fukaya Masahiro
Hokkaido Univ. School Of Medicine Sapporo
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