Studies on marine bacteria producing lytic enzymes - IX : Effect of inorganic salts on the autolysis of bacterial cells Capable of Producing Lytic Enzyme

概要

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The effect of inorganic salts on the cell lysis was investigated using strain V 37 cells at the early logarithmic phase of growth. Strain V 37 cells grown in polypeptone-yeast extract medium containing NaCl or KCl ( 0.5 M ) exhibited a remarkable lysis, when the cells were washed and suspended in 0.05 M Tris-HCl buffer (pH 7.0 ) comprising 0.5 M NaCl, KCl, LiCl,NaNO3 and NH4Cl, respectively. However, strain V 37 cells grown in the medium containing MgCl2 ( 0.5 M) showed low autolytic activity, as compared with that of the cells grown in the medium containing NaCl, KCl or MgSO4. The elevated NaCl at the concentrations from 0.1 to 2.0 M considerably decreased autolysis of strain V 37 cells. Low concentration ( 0.05 M ) of Mg2+ inactivated the autolytic activity of strain V 37 cells.
三重大学水産学部の論文
1983-10-01