Decreased Expression of Toll-like Receptor 2 and 4 on Macrophages in Experimental Severe Acute Pancreatitis
スポンサーリンク
概要
- 論文の詳細を見る
In severe acute pancreatitis (SAP), immunologic impairment in the early phase maybe linked to subsequent infectious complications that are main contributor to the highmortality. Toll-like receptors (TLRs) recognize microorganisms as the innate immunesystem, and are involved in host defense mechanism. TLR2 recognizes lipoteichoic acid(LTA) of gram-positive bacteria, and TLR4 recognizes lipopolysaccharide (LPS) ofgram-negative bacilli. This study aimed to investigate the expression of TLRs onmacrophages and their TLRs-mediated cytokine production in rat SAP. SAP wasinduced by retrograde injection of 3% sodium deoxycholate into the biliopancreaticduct in male Wistar rats. Macrophages were isolated from bronchoalveolar lavage fluid6 hours after induction of SAP. The expression of TLR2 and TLR4 was analyzed byreal-time RT-PCR and western blotting. TNF-α release from macrophages wasestimated after 4-hour stimulation of LTA or LPS. Endotoxin/bacterial translocation(E/BT) was also evaluated in this model. The expression of TLR2 (mRNA and protein)and LTA-mediated TNF-α production were significantly decreased in SAP comparedwith control. The expression of TLR4 (mRNA and protein) and LPS-mediated TNF-αproduction was also significantly decreased in SAP compared with control. E/BToccurred 18 hours after induction of SAP. These results suggest that the impairedresponsiveness to LTA and LPS of macrophages is derived from decreased expressionof TLR2 and TLR4, respectively. This suppression of immune response in the earlyphase may be implicated in the mechanism of infectious complications.
- 神戸大学医学部の論文
- 2007-01-00
神戸大学医学部 | 論文
- 人工弁置換術後死亡例の病理組織学的研究
- 実験的中枢性潰瘍における胃十二指腸粘膜アミンの動態
- 化学発癌剤の染色体レベルでの作用
- 赤血球代謝よりみた高令者術後管理に関する検討--特に2,3-DPG低下防止策について
- Minnesota Impedance Cardiographyに関する研究--波形分析の臨床的評価