Serotype Identification of Dengue Virus Isolates by an Enzyme-linked Immunosorbent Assay Employing a Protein Denaturing Agent.
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概要
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Serotypes of dengue isolates were identified successfully by an enzyme-linked immunosorbent assay (ELISA) reacting with crude viral antigens and conventional hyperimmunized anti-dengue mouse antibodies. In the ELISA, a protein denaturing agent, sodium thiocyanate (NaSCN) was employed to discriminate between serotype specific reaction and nonspecific cross-reaction among four serotypes of the virus. Antigen fractions in sucrose gradient were highly cross-reactive to both homologous and heterologous antibodies in the absence of NaSCN in the ELISA, and their serotype specificities were improved by the treatment of NaSCN in the ELISA. Fifteen out of 38 strains (ca. 38%) and 12 out of 38 strains (ca. 31.5%) of dengue isolates were recovered and identified by IFA staining and the ELISA from field specimens kept at -80℃ for 7 years, respectively.
- 長崎大学熱帯医学研究所の論文
- 1995-03-31
長崎大学熱帯医学研究所 | 論文
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