緑膿菌のピオシン型別に関する研究-5-分離株に対する型別法2種の併試特に集落型の差による産生ピオシンの作用域比較
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The methods of both Darrell-Wahba and Gillies-Govan were applied in parallel to 142 strains, excepting strain No.92 of the 143 isolates used in the reports I-III. Throughout the present study, in the case of G-G method, pyocine typing was performed on Try-ptosoy agar (Eiken) plates containing 5% defibrinated horse blood (Shiibashi). In the first experiment, 1-3 day old slant cultures originated from a la colony of each test strain were typed by the two methods during 3 weeks between the second and the third experiment informed in the last report. Each fresh la colony of indicator strains was respectively used for typing the first group of the test strains and a set of slant cultures derived from a part of the same la colony was applied to the remaining 3 groups of them. According to the G-G method (Tables 1 and 2), 120 out of 142 strains were typable into 13 types of original schema and remaining 22 were unclassifiable, whereas only 26 were typable and 116 were unclassifiable by the D-W method (Tables 3-6). The results obtained by the D-W method can be summerized as follows: 11 strains of Type B1, 5 of Type B2, 1 of Type B3, 6 of Type L, 3 of non-producer, and 20 strains of No.38 type in Table 3, 11 of No.104 type in 4, 9 of No.27 type in 3, 8 of No.4 type in 4, 4 strains each of No.2, 101, 77 and 106 types in Table 4, 3 strains each of No.66:74:49 (Tables 4 and 6), No.80:94:90 (4, 6), No.84:139:35 (6) and No.52:159:26 (6) types, 2 strains each of No.123:53 (4, 6), No.42:143 (4), No.78:147 (4, 5), No.121:59 (4, 6), No.103:57 (5, 6), No.108:18 (6) and No.71:72 (6) types, and 1 strain each of other 26 patterns. Reproducibility of typing results former, present and further experiments performed by the two methods will be compared with one another and discussed in the near future. Secondly, fresh la and sm colonies of 54 test strains which could be selected from all of 142 isolates by applying the simple isolation technique on nutrient agar plate were subjected to type by the two methods in parallel, using a la colony of each indicator isolated just before the use, for the period between the third and the fourth experiment informed in the last report. As shown in Tables 7-10, differences of the inhibition patterns between la and sm colonies of the test strains were observed in 19 out of 54 strains by the G-G method (Table 8) and 25 strains by D-W method (Table 10). It can be said that different patterns of inhibition were also observable on la and sm colonies of isolates, in addition to the same observation obtained by using indicators and type strains as producers in the third experiment stated in the last report. To compare the results obtained above by the two methods using indicators of the same number, strains W9-W12 for the D-W method were omitted for trial from Table 10 like "Mayo types" of Zabransky and Day. Even so, la and sm colonies of 22 strains excluding No.4, 99 and 56 from 25 strains in Table 10 still show different inhibitions to some of indicators. Next the comparable part of the results standing in the same colonial relationship of producer and indicator to this experiment were selected from Tables 7-10 of the last report and added to the number of the strains mentioned above. Finally, differences of the inhibition patterns between la and sm colonies are seen in 22 out of 65 strains by the G-G method and 27 by modified D-W method. These observations indicate that colonial types of producers may be more effective in pyocine typing by the D-W method than that by the G-G method.
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