Changes of Proteoglycan Expression and Glycosaminoglycan Constituents in the Intervillous Space of the Pregnancy-Induced Hypertension Placenta
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The changes in proteoglycan (PG) expression and glycosaminoglycan (GAG) constituents in theintervillous space of the pregnancy-induced hypertension (PIR) placenta were investigated. PGs and GAGs werepurified from the extract of the placental intervillous space by the DEAE-Sephacel column and salt-concentrationgradient method. and the GAG sugar chains were released by the actinase and cellulase treatments. Thesugar chains from the placentas of normal pregnancy and PIR were compared by cellulose-acetate membraneelectrophoresis. No difference was observed in the expressions of hyaluronic acid. heparan sulfate, and chondroitinsulfate. but a clear increase in the expression of dermatan sulfate (DS) in the placenta of the PIR was confirmed.An increase of the DS that specifically activates anticoagulants can be a body reaction to counteract the hemostaticcondition observed in PIR.
- 2008-03-31
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