Effect of Exogenous Proteoglycans on Chondrogenesis of Bone Marrow Mesenchymal Stem Cells

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Proteoglycans are one of the most important components of the extracellular matrix in the cartilageand the levels of proteoglycans. such as versican and aggrecan, increase during chondrogenesis. The purpose ofthis study was to investigate the effect of exogenous proteoglycans from salmon nasal cartilage on chondrogenesisof mesenchymal stem cells. Mesenchymal stem cells derived from bone marrow aspiration of rabbit femurs wereinduced to chondrogenic lineage using a pellet culture technique. Pellets were cultured in the medium with orwithout cell growth factors. with or without proteoglycans. or a combination of these agents. Pellets treated with cellgrowth factors became hypertrophic and showed lacuna formation. and synthesis of cartilage matrix was recognizedhistologically. The expression of type II collagen and aggrecan mRNA were decreased in pellets incubated with acombination of cell growth factors and proteoglycans, compared to those incubated with only cell growth factors.Exogenous proteoglycans may down-regulate the expression of cartilage-specific mRNA directly, or may interactwith growth factors in the culture medium. As the increase of glycoprotein during chondrogenesis is important fordetermining the direction and degree of differentiation. exogenous proteoglycans may have a similar effect.
2008-03-31