Quantitative Evaluation of the Inactivation of Human Immunodeficiency Virus (HIV) by Antiseptics for the Oral Cavity
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概要
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Antiseptics used for the oral cavity were tested for the inactivation of human immunodeficiency virus (HIV). A quantitative bioassay system with a HTLV-Ⅰ - carrying human T-cell line, MT-4 was used to evaluate virus inactivation. The tested compounds were as follows: povidone-iodine; benzethonium chloride; benzalconium chloride; chlorhexidine digluconate; methylrosaniline chloride; benzalconium chloride; chlorhexidine digluconate; methylrosaniline chloride; and ethacridine lactate. These compounds were tested at final concentrations of 1.0%, 0.1%, 0.01% and 0.001%, in the presence or absence of 10% fetal calf serum. The immunofluorescence assay for the HIV antigen showed that povidone-iodine, benzethonium chloride, and chlorhexidine digluconate were effective in the inactivation of HIV at concentrations for oral use. However, in the presence of protein, benzethonium chloride and chlorhexidine digluconate were ineffective at the indicated concentrations for oral use. Although benzalconium chloride was effective at 0.1%, this concentration cannot be used orally. Ethacridine lacate and methylrosaniline chloride were not effective at non-cytotoxic concentrations. The inhibitory effect of povidone-iodine was further tested by a HIV-specific plaque forming assay. The virus treated with concentrations higher than 0.1% of providone-iodine inactivator of HIV for use as an oral actiseptic in routine dental practice.
- Yamaguchi University Graduate School of Medicineの論文