Imaging of cerebrospinal fluid space and movement in mice using near infrared fluorescence
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概要
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We developed an optical method for imaging the cerebrospinal fluid (CSF) space and the movement of CSF in mice using a near infraredfluorescence imaging methodology. Indocyanine green bound to high-density lipoprotein (ICG-HDL) was injected into the lumber subarachnoidspace of nude mice. The time course of CSF movement was followed over 48 h. The imaging system was configured for epi-fluorescencemeasurements at near infrared wavelengths using the illumination light and narrow band excitation filtration with central wavelength of755 nm. Emission light was filtered with a longpass filter with a cutoff at 798 nm. After the injection of ICG-HDL, a strong fluorescencesignal clearly delineated the cisterna magna, bilateral supracerebellar/cerebello-pontine angle cistern, and interhemispheric cistern. Much ofthe fluorescent tracer was washed out within 24 h after the injection. This study has therefore demonstrated that an optical method, employingnear infrared fluorescence imaging, can determine the CSF space and the movement of CSF in nude mice.
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