Thermolysin Activation by Water-miscible Organic Solvents in Aqueous-organic One-phase Reaction System is not Dominated by Their Physical or Physicochemical Parameters
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Thirteen water-miscible organic solvents, except for alcohols, with infinite solubility in water; and two physical and eighteen physicochemical parameters were adopted for enzymatic peptide synthesis in water-organic one-phase reaction system. Results showed that the activation of thermolysin by water-miscible organic solvents has striking similarity with that of water-immiscible solvents. They exhibited common activation profile and kinetic mechanism. It was demonstrated that activation is not controlled by any of the solvent parameters-suggesting the involvement of specific atoms and/or groups.
- 信州大学農学部の論文
- 1998-09-30
信州大学農学部 | 論文
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