Immunoassay using ion selective electrode and protein pendant liposomes

概要

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Immunoassay for an anti-human IgG antibody and a human IgG is presented. Thin-layer potentiometry, i.e., a combinationof fluoride ion selective electrode and silver chloride coated silver-plate reference electrode was used to monitor fluoride release from the multilamellar liposome. Human IgG was attached to the surface of liposome membrane by the use of cross-linking reagent N-hydroxysuccinimidyl3-(2-pyridyldithio)propionate (SPDP). The antigen/antibody/complement reaction triggered the formation of "channel-like" holes across the liposome membrane which enabled entrapped fluoride anion flow through the hole. The fluoride ion release was specific for anti-human lgG and depended on the presence of complement. By the use of human IgG pendant liposomes,determination of about 10^-14 mol of anti-human IgG (rabbit) and 10^-14 mol human IgG was feasible.
日本分析化学会の論文