Isolation of thermophilic L-lactic acid producing bacteria showing homo-fermentative manner under high aeration condition(ENVIRONMENTAL BIOTECHNOLOGY)
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概要
- 論文の詳細を見る
By applying non-sterile open fermentation of food waste, various thermotolerant L-lactic acid-producing bacteria were isolated and identified. The predominant bacterial isolates showing higher accumulation of L-lactic acid belong to 3 groups of Bacillus coagulans, according to their 16S rRNA gene sequence similarities. B. coagulans strains M21 and M36 produced high amounts of L-lactic acid of high optical purity and lactic acid selectivity in model kitchen refuse medium and glucose-yeast extract-peptone medium. Other thermotolerant isolates resembling to Bacillus humi, B. ruris, B. subtilis, B. niacini and B. soli were also identified. These bacteria produced low amounts of L-lactic acid of more than 99% optical purity. All isolated strains showed the highest growth rate at temperatures around 55-60℃. They showed unique responses to various oxygen supply conditions. The majority of isolates produced L-lactic acid at a low overall oxygen transfer coefficient (K_Lα); however, acetic acid was produced instead of L-lactic acid at a high K_Lα. B. coagulans M21 was the only strain that produced high, consistent, and reproducible amounts of optically pure L-lactic acid (>99% optical purity) under high and low K_Lα conditions in a homo-fermentative manner.
- 公益社団法人日本生物工学会の論文
著者
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Yoshino Satoshi
Department of Cardiology, Respiratory and Metabolic medicine, Graduate School of Medicine, Kagoshima
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Tongpim Saowanit
Department Of Biotechnology Graduate School Of Engineering Osaka University:(present Address)departm
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Taniguchi Masayuki
Department Of Cardiology Jikei University School Of Medicine Daisan Hospital
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Sakai Kenji
Department Of Applied Chemistry Faculty Of Engineering Oita University
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Taniguchi Masayuki
Department of Materials Science and Technology, Faculty of Engineering, Niigata University
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Tashiro Yukihiro
Department of Bioscience and Biotechnology, Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University
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Poudel Pramod
Department of Bioscience and Biotechnology, Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University
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Meidong Ratchanu
Department of Microbiology, Faculty of Science, Khon Kaen University
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Okugawa Yuki
Department of Bioscience and Biotechnology, Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University
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Tongpim Saowanit
Department of Microbiology, Faculty of Science, Khon Kaen University
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Yoshino Satoshi
Department of Bioscience and Biotechnology, Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University
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