落花生におけるCa45のミクロオートグラフ作製法並びにその分布に就いて

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Peanut plants of the Virginia Runner Variety (Chiba 74) were grown in sand culture with the root and fruit zones isolated from each other as shown in Fig. 1. The top of each pot, which held the root of the plant, was placed 1 inch above the surface of the sand in the fruiting glass jug. When the gynophore reached the surface of the jug, (Ca)^<45>-(Cl)_2 was added, at the rate of 10μc, to each jug containing250 gr. of sand. As regards the technique for microautoradiograph, the stripping emulsion method was tried by using fuji autoradiographic plate ET-2E in a dark room. And the slides were exposed to β-ray of (Ca)^<45> from 8 to 128 days in the light tight box. The best exposure time was about 2 months in the case of histological section of 25μ. And it was important to avoid overexposure, since this caused a loss of resolution as shown in Fig. 2 (a-d). The relationship between the deposition of (Ca)^<45> in peanut and the anatomical structure was presented in Fig. 2 (e-i). In the autoradiogram of the longitudinal sections of 10,30-day-old fruit, it was noted that the extent and nature of the (Ca)^<45> deposition was characteristic and was a reflection of the particular fruit structure, as follows; A, the (Ca)^<45>-containing compounds were relatively concentrated in the cortex of the gynophore, especially in the single layer of endodermis. B, within the pod, they were found to be in the quantities in the tracheid and epidermis parts of the shell, while in the seed or seed coat scarcely any (Ca)^<45> was found. It seemed that from the basal region of shell a considerable amount of (Ca)^<45> entered the terminal one during the development of the fruit.
神戸大学の論文

著者

水野進
兵庫農科大学

落花生におけるCa45のミクロオートグラフ作製法並びにその分布に就いて

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