ヒト白血病細胞HL-60におけるp21waf1の発現亢進:12-o-tetradecanoylphorbol 13-acetateによる分化およびアポトーシス誘導との関連

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TPA (12-o-tetradecanoylphorbol 13-acetate) induces monocyte/macrophage differentiation and adhesion in the human leukemic HL-60 cell line, and cyclin D1 and p21waf1 (cell cycle control genes) are overexpressed in TPA-treated adherent HL-60. It is also known that TPA induces apoptosis of the cell line. In this study, to investigate the relationship between differentiation and/or apoptosis induction and cell cycle control genes, we analyzed the expressions of p21waf1 and cyclin D1 mRNA in adherent and floating cells separately. Using flow cytometry, there was an increase in the apoptosis of floating cells when TPA treatment was applied. Quantitative analysis using real-time polymerase chain reaction revealed that p21waf1 and cyclin D1 expression was increased in both the floating and adherent cells. There was an increase in apoptosis of floating cells 3 h post-TPA treatment, and by this time the expression of p21waf1 had already significantly increased. Adherent cells were observed 12 h after TPA treatment, and by this time the expression of p21waf1 had already increased. These observations suggest that the increased expression of p21waf1 may play a role in the induction of apoptosis by TPA in HL-60 cells.
2014-03-31