S021021 前十字靭帯再建用のウシ由来無細胞腱の開発([S02102]人工臓器におけるバイオメカニクス(2))

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Autologous tendons or bone-patellar tendon-bone grafts are clinically used for reconstruction of anterior cruciate ligaments, because of unsatisfactory durability of synthetic materials. The aims of this study are to realize decellularization of 16-cm long xenogeneic tendons which have a potential to guide regeneration, and to investigate influences of decellularization on tissue mechanical properties. Bovine tendons were decellularized by combination of pulsatile flow-and-pressure circulation of 1 wt% deoxycholic acid and microwave radiation. Then, the tissues were additionally treated with endonuclease. Influences of decellularization on durability was investigated by a cyclic tensile test under 0-100 N loads. When the tissues were not ruptured, ultimate strength, elastic modulus, and ultimate strain were examined by a uniaxial tensile test. DNA of 16-cm long bovine tendons was successfully removed over 99.99% by our decellularization technology. Non-treated and decellularized tendons were not ruptured in 1000 cycles' tensile durability tests. After the cyclic tests, ultimate stress, elastic modulus, and ultimate strain of the decellularized tendons were comparable to those of non-treated tendons. We here reported, to our knowledge, the first successful decellularization of 16-cm long tendons with preserving mechanical integrity.
2011-09-11