J024051 表面改質を施した基材上での軟骨細胞の培養と流体せん断力による複合刺激の効果([J02405]バイオにおける流れと熱・物質移動(5))

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概要

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• Recently, various studies have investigated cartilage tissue engineering. Implantation of chondrocytes isolated from patients and expanded in number in vitro is being used to treat patients with cartilage injuries. However, chondrocytes de-differentiate during culture with several passages, and the cartilage regenerated by implantation of de-differentiated chondrocytes may be suboptimal. Therefore, it is important to establish the method for culturing chondrocytes to re-differentiate and proliferate. A hydrodynamic environment is beneficial for culturing chondrocytes and bio-modification of culture substrate is also beneficial to improve cell cytoskeleton; however, collaborative effect of both the hydrodynamic effect and bio-modification were not studied. In this study, we investigated the synergetic effect of the fluid shear stress and the bio-modification of culture substrate. Passaged bovine articular chondrocytes were cultured in monolayers to sub-confluency. After 24 h of growth arrest, cells on the gelatin-coated glass were exposed to the fluid shear stress. Cell number and cell morphology were measured to evaluate the effect of fluid shear stress and bio-modification on the cell proliferation and differentiation.

• 一般社団法人日本機械学会の論文
• 2011-09-11

著者

• 小茂 鳥潤

  慶應義塾大学理工学部機械工学

• 大井 俊史

  慶應義塾大学

• 小茂 鳥潤

  慶應義塾大学理工学部機械工学科

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