P6 HPLC/パルスアンペロメトリーによる二糖類超音波分解反応機構の解析(ポスターセッション)

概要

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High performance liquid chromatography (HPLC) coupled with triple pulsed amperometric detection (TPAD) was developed for the determination of trace carbohydrates with high precision and sensitivities. The method was applied for the determination of the degradation products in hydrolysis reaction of disaccharides. The disaccharide sucrose can be hydrolyzed to the two monosaccharides, glucose and fructose with ratio of 1:1 in strong acidic condition. This reaction was not identified in a basic media (0.1M NaOH solution). Under ultrasound irradiation with the frequency of 45KHz, however, sucrose was rapidly decomposed even in a basic media. Results form HPLC analysis indicated that sucrose was decomposed into glucose and fructose under the ultrasonic irradiation, but the latter product yielded higher fraction in the mixture. It thus supposed that the produced glucose was further isomerizes to fructose under the ultrasonic irradiation. Although the mechanism of sucrose isomerization is speculative, evidence shows that glucose was converted into fructose as a function of ultrasound irradiation time.
日本ソノケミストリー学会の論文
2006-10-27