A1 二酸化チタン/超音波法による細胞障害
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概要
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Titanium dioxide (TiO_2) is known as photocatalytic material, and it generate reactive oxygen species (ROS) according to be excited by ultraviolet rays (UV) under 380nm. Those ROS generated from TiO_2 could sterilize E coli, and have cytotoxic effect on mammalian cancer cells. Previously, we reported the generation of OH radicals from TiO_2 particles excited by ultrasonic (US) irradiation, not UV. Furthermore, the TiO_2 excited by US exhibited decompose activity on E coli and chemical substances. In this study, we report the cytotoxic effect of US in the presence of TiO_2 particles in cell culture media. To estimate the cytotoxic effect, we measured lactase dehydrogenase (LDH) activity leaked from damaged cell numbers after US irradiation. Immediately after US irradiation in the presence of TiO_2 particles, the LDH activity exhibited high, indicating TiO_2 with US irradiation caused damage on cell membrane stability. Furthermore, we confirmed the cell membrane damage by flowcytometry assay with Annexin V-FITC/Propidium iodide (PI) stain method, Annexin V-negative/PI positive cells were dominant immediately after US irradiation in the presence of TiO_2 particles.
- 日本ソノケミストリー学会の論文
- 2006-10-27
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