Relationship between Escherichia coli AppA phytase's thermostability and salt bridges(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

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概要

• 論文の詳細を見る

• In order to study on the relationship between Escherichia coli AppA phytase's thermostability and salt bridges, and indicate an effective technical route of which factor to think about and where to modify at AppA for enhancing its thermostability, a salt bridge subtraction mutant E31Q and a salt bridge addition mutant Q307D were constructed by site-directed mutagenesis. The residual activities of the wild-type AppA phytase, E31Q and Q307D were 31.42%, 17.46%, and 40.57%, respectively, after being heated at 80℃ for 10 min. The salt bridge subtraction mutant E31Q showed 13.96% thermostability decreasement, and the salt bridge addition mutant Q307D showed 9.15% thermostability enhancement than the wild-type both without the pH and temperature optimum changed. It proved salt bridges play a key role in E. coli AppA phytase's thermostability and the α/β-domain of AppA may be sensitive to heat. Salt bridges and the α/β-domain of AppA should have high priority to think about to enhance AppA's thermostability for commercial application. Besides, molecular dynamics simulation was used for salt bridges analysis.

• 公益社団法人日本生物工学会の論文
• 2013-06-00

著者

• CAO Yi

  Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Science,

• Cao Yu

  Microbiology And Metabolic Engineering Key Laboratory Of Sichuan Province College Of Life Science Si

• Xu Hui

  Microbiology And Metabolic Engineering Key Laboratory Of Sichuan Province College Of Life Science Si

• Cao Yu

  Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Science, Sichuan University

• Cao Yi

  Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Science, Sichuan University

• Fei Baojin

  Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Science, Sichuan University

• Zhang Feiwei

  Chengdu Institute of Biological Products Co., Ltd.

• Li Xinran

  Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Science, Sichuan University

• Ma Shuhan

  Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Science, Sichuan University

• Xie Jie

  Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Science, Sichuan University

• Qiao Dairong

  Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Science, Sichuan University

• Xu Hui

  Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Science, Sichuan University

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