Use of reporter gus-gene to study the colonization of rice roots by Azospirillum lipoferum
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概要
- 論文の詳細を見る
Azospirillum lip oferum gus-marked strain T 137-1 was constructed by Tn5 mutagenesis using E. coli S 17-1::mTn5SSgusA 20. The frequency of Tn5 mutagenesis was 2 x 10^<-7>. The transconjugant retained most of the properties of the wild strain as confirmed by biochemical tests and the reaction of the antiserum to A. lipoferum. The colonization ability of the gus-marked strain of Azospirillum was studied in growth pouches in a greenhouse at 5, 10, 20 and 30 days after inoculation (DAI). At 5 DAI, Azospirillum strains abundantly colonized the root surface. At 20 and 30 DAI, Azospirillum strains were still observed on young lateral roots and root hairs but the attachment sites were sparse and highly variable between the replicates Abbreviation: DAI-days after inoculation, ARA-acetylene reduction activity.
- 日本土壌微生物学会の論文
- 1999-03-01
著者
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Gaali Eisa
National Institute Of Agrobiological Resources
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Akao Shoichiro
National Institute Of Agrobiological Resources
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CHEBOTAR Vladimir
National Institute of Agrobiological Resources
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NAKAYAMA Yasuji
National Institute of Agrobiological Resources
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KANG Ui
National Institute of Agrobiological Resources
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Akao Shoichiro
National Institure Of Agrobiological Resources
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Chebotar Vladimir
National Institute Of Agrobiological Resources:all-russia Institute For Agricultural Microbiology
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Kang Ui
National Institute Of Agrobiological Resources:national Yeongnam Agricultural Experiment Station
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- Use of reporter gus-gene to study the colonization of rice roots by Azospirillum lipoferum
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