Nuclear localization of reporter genes activated by curved DNA(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)
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概要
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Curved DNA structures with a left-handed superhelical conformation can activate eukaryotic transcription. Mechanistically, these structures favor binding to histone cores and can function as a docking site for sliding nucleosomes. Thus, promoters with this kind of curved DNA can adopt a more open structure, facilitating transcription initiation. However, whether the curved DNA segment can affect localization of a reporter gene is an open question. Localization of a gene in the nucleus often plays an important role in its expression and this phenomenon may also have a curved DNA-dependent mechanism. We examined this issue in transient and stable assay systems using a 180-bp synthetic curved DNA with a lefthanded superhelical conformation. The results clearly showed that curved DNA of this kind does not have a property to deliver reporter constructs to nuclear positions that are preferable for transcription. We also identify the spatial location to which electroporation delivers a reporter plasmid in the nucleus.
- 公益社団法人日本生物工学会の論文
著者
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Tanabe Hideyuki
Department Of Biosystems Science School Of Advanced Sciences The Graduate University For Advanced St
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Kimura Hajime
Major In Integrative Bioscience And Biomedical Engineering Graduate School Of Science And Engineering Waseda University
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Udagawa Koji
Major in Integrative Bioscience and Biomedical Engineering, Graduate School of Science and Engineering, Waseda University
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Ohyama Takashi
Major in Integrative Bioscience and Biomedical Engineering, Graduate School of Science and Engineering, Waseda University
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Udagawa Koji
Major In Integrative Bioscience And Biomedical Engineering Graduate School Of Science And Engineering Waseda University
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Ohyama Takashi
Major In Integrative Bioscience And Biomedical Engineering Graduate School Of Science And Engineering Waseda University:department Of Biology Faculty Of Education And Integrated Arts And Sciences Waseda University
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Ohyama Takashi
Major In Integrative Bioscience And Biomedical Engineering Graduate School Of Science And Engineering Waseda University
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