Mapping quantitative trait loci controlling root length in rice seedlings grown with low or sufficient NH_4^+ supply using backcross recombinant lines derived from a cross between Oryza sativa L. and Oryza glaberrima Steud.(Plant Nutrition)
スポンサーリンク
概要
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To understand genetic adaptation of rice to environmental nitrogen status, this study mapped and verified the quantitative trait locus/loci for root elongation in response to exogenous NH_4^+ concentration. Significant inhibition of root elongation by increasing NH_4^+ concentration in hydroponic culture was observed in seedlings of Oryza sativa cv Taichung 65 (japonica variety) but not in O. glaberrima line IRGC 104038. A total of 161 backcross recombinant lines between these species were employed to map the quantitative trait locus/loci controlling root length of seedlings grown hydroponically with a low (5μM) or sufficient NH_4^+ (500μM) supply. Five quantitative trait locus/loci were detected: two on chromosome 1 and three singles on chromosomes 2, 3, and 6. The quantitative trait locus/loci qRL1.1 was significant under sufficient NH_4^+ supply. Progeny analysis using heterogeneous inbred-type family nearly isogenic lines (HIF-NILs) for the candidate region of qRL1.1 revealed that seminal roots of IRGC 104038-fixed genotypes were significantly longer (12.4 or 11.6%) than those of Taichung 65-fixed genotypes when grown with 500μM NH_4^+, but not without or with 5μM NH_4^+. qRL1.1 was confirmed and delimited within a 4-Mbp-long region on the long-arm region of chromosome 1. Comparative mapping with genes for NH_4^+ uptake and nitrogen metabolism showed the apparent location of the aspartate aminotransferase gene (OsAAT2) within the candidate region of qRL1.1. Together, the results suggest that qRL1.1 is an adaptive quantitative trait locus/loci for rice root elongation in response to sufficient supply of external NH_4^+ and OsAAT2 may be a candidate gene responsible for qRL1.1.
著者
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YAMAYA Tomoyuki
Graduate School of Agricultural Science, Tohoku University
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Yamaya Tomoyuki
Graduate School Of Agric. Sci. Tohoku Univ.
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Obara Mitsuhiro
Graduate School Of Agric. Sci. Tohoku Univ.
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HAYAKAWA Toshihiko
Graduate School of Agricultural Science, Tohoku University
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Hayakawa Toshihiko
Graduate School Of Agric. Sci. Tohoku Univ.
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Hayakawa Toshihiko
Graduate School Of Agricultural Science Tohoku University
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TAKEDA Takumi
Iwate Biotechnology Research Center
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