〔報文〕食事性L-Arginineの骨構造および骨代謝関連遺伝子発現調節に対する影響

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The objective of the present study was to evaluate the beneficial effects of dietary L-Arginine as a bone-protective factor based on the bone microstructural properties and the mRNA expression levels of genes related to the bone metabolism and L-Arginine level in ovariectomized 24-month-old female Wistar rats. The rats were allowed free access to water containing 0%(OVX-C)or 2% L-Arginine(OVX-Arg)as their only drinking water for 13 weeks. All rats were fed a commercial stock diet ad libitum during the experimental period. No significant differences were observed in the body weights, the results of blood biochemical analyses, or the organ weights in comparisons between the OVX-C and OVX-Arg groups. The OVX-Arg group showed 1)higher(but not significantly higher)values in bone mineral density(BMD)and the bone microstructure analysis, 2)significantly higher mRNA expression levels of BMP-2, RANKL, Insulin-like growth factor-1(IGF-1), and CAT-1, which is a transporter of L-Arg, in the rat femur, 3)relatively higher mRNA expression levels of estrogen receptor- (ER- )and endotherial nitric oxide synthase(eNOS), compared to the OVX-C group. These results suggest that dietary L-Arginine promotes bone remodeling and bone formation by increasing the ER- -eNOS-NO signaling pathway in 24-month-old, ovariectomized female Wistar rats.
2011-12-01