Growth and poliovirus production of Vero cells on a novel microcarrier with artificial cell adhesive protein under serum-free conditions(METHODS)
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概要
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A microcarrier is used for the three-dimensional (3D) culture of adhesion-dependent mammalian cells. We developed a novel microcarrier by binding ProNectin F, an artificial cell adhesive protein synthesized by genetically engineered Escherichia coil to a polyacrylic superabsorbent polymer. The microcarrier is characterized by containing no animal-derived components. The serum-free culture of Vero cells for vaccine production using the microcarrier increased the number of Vero cells by approximately 30% compared with the existing dextran beads coated with porcine Type I collagen, which resulted in approximately a 30% to 40% increase in the infectivity titer of the Sabin 2 strain of poliovirus. These results suggested that the developed microcarrier should be unprecedented in permitting high-yield vaccine production by means of a serum-free culture.
著者
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Sato Shigehiro
Department Of Applied Chemistry And Biotechnology Faculty Of Engineering Niigata Institute Of Techno
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KUROKAWA MASATO
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University
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Kurokawa Masato
Department Of Microbiology School Of Medicine Iwate Medical University:medical Care Intracorporation
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Kurokawa Masato
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Kurokawa Masato
Department of Electrical Engineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan
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