Novel screening system for protein-protein interactions by bimolecular fluorescence complementation in Saccharomyces cerevisiae(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
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概要
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For high-throughput screening of protein-protein interactions, we have developed a novel yeast screening system using Bimolecular fluorescence complementation (BiFC). Two yeast plasmids, in which genes of heterodimerized peptides LZA and LZB were each fused with those of non-fluorescent half fragments of Kusabira-Green mutant (mKG2), were transformed into a- and α-type yeast, respectively. Mating of them gave a library, which was screened by following green fluorescence resulted from LZA-LZB interaction. The method showed potential ability to detect the positive clones from a model library, in which green-fluorescent and non-fluorescent yeast was mixed in a ratio of 1:675.
著者
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Miyawaki Atsushi
Laboratory for Cell Function and Dynamics, Advanced Technology Development Center, RIKEN
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Kojima Takaaki
Laboratory Of Chemical Biology Department Of Biological Science Graduate School Of Science Osaka Pre
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Tsumuraya Takeshi
Laboratory For Cell Function And Dynamics Advanced Technology Development Group Brain Science Instit
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Karasawa Satoshi
Laboratory for Cell Function and Dynamics, Advanced Technology Development Group, Brain Science Inst
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Fujii Ikuo
Laboratory of Chemical Biology, Department of Biological Science, Graduate School of Science, Osaka
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Fujii Ikuo
Laboratory Of Chemical Biology Department Of Biological Science Graduate School Of Science Osaka Pre
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Miyawaki Atsushi
Laboratory For Cell Function And Dynamics Advanced Technology Development Group Brain Science Instit
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Miyawaki Atsushi
Laboratory For Cell Function And Dynamics Advanced Technology Development Center Brain Science Insti
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Karasawa Satoshi
Laboratory For Cell Function And Dynamics Advanced Technology Development Group Brain Science Instit
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