Trans-acting RNAs as molecular probes for monitoring time-dependent structural change of an RNA complex adapting two structures(METHODS)
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概要
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As dynamic structural changes are pivotal for the functions of some classes of RNA molecule, it is important to develop methods to monitor structural changes in RNA in a time-dependent manner without chemical modification. Based on previous reports that trans-acting RNAs can be used as probes for analysis and control of 3D structures of target RNAs, we applied this method to monitor time-dependent structural changes in RNA. We designed and performed a proof-of-principle study using a simple model RNA complex that adopts two different structures as a target. The time-dependent structural changes in the target RNA were successfully monitored using two trans-acting RNAs, which stably form a ternary complex with the bimolecular target RNA and act as a catalyst to join two RNA fragments of the target complex, respectively.
著者
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Ikawa Yoshiya
Department Of Chemistry And Biochemistry Graduate School Of Engineering Kyushu University
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Maeda Yuri
Department Of Obstetrics And Gynecology Saga Medical School
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Furuta Hiroyuki
Department Of Chemical Science And Engineering Faculty Of Engineering
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Ikawa Yoshiya
Department Of Chemistry And Biochemistry Graduate School Of Engineering Kyushu University:internatio
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Furuta Hiroyuki
Department Of Chemistry And Biochemistry Graduate School Of Engineering Kyushu University:internatio
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Maeda Yuri
Department Of Chemistry And Biochemistry Graduate School Of Engineering Kyushu University
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Furuta Hiroyuki
Department Of Applied Chemistry Oita University
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