236 Development of An Overall Mechanical Model For Osteoblast Bone Cells

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Many individuals and in particular elderly suffer from bone diseases like Osteoporosis, and bone fracture or cracking are problems which many athletes as well as ordinary people tackle, since they take rather long time to get treated. It has been observed that exposing bone cells to mechanical vibration with special frequencies could cause dramatic variations in Osteoblast cell proliferation rate. Previous works proposed the phenomenon is driven by the cell mechanical sensors, by which it senses mechanical manipulations. On the other hand, treatments that employ ultrasound in order to increase the repair rate of bones are getting popular. Hence modeling cells against loads in order to predict their response to mechanical waves is indispensable to treat diseases using mechanical vibration. Also using a more advanced model concerning the interaction of cells with their surrounding, it would be possible to predict and analyze the effect of different body activities on the cells, that could be useful yielding a more detailed understanding of Osteoblast life. In this study we developed an overall mechanical model using a novel method, taking into account the uniaxial instant and viscoelastic parameters for load bearing members of a single Osteoblast cell (See Fig.A1). The model was considered to be overall, since the whole body of the cell was considered as a single viscoelastic material, rather than a combination of its load bearing members such as membrane and cytoskeletal fibers. Osteoblast cells have been seeded on an extra-soft gel string, whose mechanical characteristics had been calibrated, and the string has been stretched, while being observed under a microscope. In this way, response of a single cell has been monitored during the load application period. This setup then has been modeled by an analysis software, in which the so called gel plate was modeled as an elastic body, using results that had been obtained from gel calibration tests. Cell load-bearing members were modeled initially as a member with unidentified mechanical properties. A trial and error method was used in order to approach a well-fit relationship between the cell deflection, observed during the experiment, and cell overall force. A creep test and a stepwise tensile test were conducted on the cell. Fig.A2 depicts strain time histories that have been obtained from the creep test, and Fig.A3 is the load pattern that was applied to the stepwise tensile test.
2009-08-03

236 Development of An Overall Mechanical Model For Osteoblast Bone Cells

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