P-119 Flocculation phenomenon of a mutant flocculent Saccharomyces cerevisiae strain : effects of metal ions, sugars, temperature, pH, protein-denaturants and enzyme treatments

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概要

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• The flocculation mechanism of a stable mutant flocculent yeast strain Saccharomyces cerevisiae KRM-1 was quantitatively investigated for its potential industrial interest. It was found that the mutant flocculent strain was NewFlo phenotype by means of sugar inhibition test. The flocculation was completely inhibited by treatment with proteinase K, protein-denaturants and carbohydrate modifier. The absence of calcium ions significantly inhibited the flocculation, indicating that Ca^<2+> was specifically required for flocculation. The flocculation was stable when temperature below 70℃ and pH was in the range of 3.0-6.0. The results are expected to help developing strategies to control the mutant flocculent yeast better for future large-scale industrial ethanol fermentation.

• 一般社団法人日本エネルギー学会の論文
• 2010-01-20

著者

• Shirai Yoshihito

  Kyushu Institute of Technology

• Wakisaka Minato

  Kyushu Institute Of Technology

• Ma Kedong

  Kyushu Institute of Technology

• Sakai Kenji

  Kyushu University

関連論文

• EN-O11 Chemical recycling of polyhydroxyalkanoates as a method towards sustainable development(Section VI Environmental Biotechnology)
• P-119 Flocculation phenomenon of a mutant flocculent Saccharomyces cerevisiae strain : effects of metal ions, sugars, temperature, pH, protein-denaturants and enzyme treatments

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