透析アミロイドーシスの発症機序に関する研究 : 血清リソゾーム酵素の関与

概要

論文の詳細を見る
The relationship between dialysis associated amyloidosis and lysosomal enzyme were studied in: 12 cases of hemodialysis (HD) patients with amyloidosis, 6 cases of HD patients without amyloidosis, 13 cases of continuous ambulatory peritoneal dialysis (CAPD) patients, 7 cases of chronic renal failure (CRF) patients without HD, and 7 control subjects. The serum levels of acid phosphatase, glucuronidase, glucosidase, and dipeptidyl aminopeptidase were measured in each group. The β_2-microglobulin (β_2MG) modification by lysosomal enzymes was tested by immunoblotting using an anti-β_2MG antibody after electrophoresis. The serum levels of lysosomal enzymes in HD patients were higher than that in the other groups. The rising ratio of enzyme activation was extremely high in the amyloidosis group under 50 years old. Moreover, β_2MG modification was demonstrated both by the addition of lysosomal enzymes to the serum of HD patient without amyloidosis, and by the incubation of the serum of HD patients with amyloidosis during hemodialysis. From these results, the activation of lysosomal enzymes caused by hemodialysis is considered to be related to the formation of dialysis associated amyloidosis.
1996-12-25