近位尿細管における人工クロライドチャンネルの発現と容量およびイオン輸送への影響

概要

論文の詳細を見る
In an attempt to clarify the mechanism of fluid and ion movement driven by chloride (Cl) conductance, we studied the effect of expressing a Cl channel forming peptide in the luminal membrane of micro-perfused rabbit renal proximal tubules. An increase in Cl conductance, as indicated by a Cl-dependent increase in apical membrane voltage (Va), was confirmed after the peptide (NK_4-M2GlyR) was perfused. In the presence of the peptide, reabsorption of fluid (Jv) was dramatically increased, a part of which was inhibited by HgCl_2. Concomitantly reabsorption of Na (J_<Na>) and Ca (J_<Ca>) was significantly increased. We propose that introduction of the new Cl conductance in the luminal membrane leads to a coordinated efflux of water across the membrane and an increase in cation translocation via paracellular pathway, resulting in an increase in Jv. This novel method is useful to address the mechanism of fluid transport driven by the Cl gradient.
東京女子医科大学の論文
2003-10-25