海産微細藻テトラセルミスのグルコースを炭素源とする従属栄養培養

概要

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Six strains of Tetraselmis including T. tetrathele, T. chui, T. alacris, T. gracilis, T. suecica Spain and T. suecica Brasil were cultured in sea water (salinity 27%) supplemented with 80 mg of ammonium sulfate, 16 mg of calcium perphosphate and 4 mg of Clewat 32 per 1 with or without 100 mM glucose under 14 hr illumination(3,000 lux)/day. All the strains except T. alacris grew well in the medium with glucose. Especially, the growth of T. tetrathele and T. chui was greatly enhanced by addition of glucose, showing the rates of 0.94 and 0.93 doublings (dbl)/day,respectively,in their logarithmic phase. When cultured in the dark, their growth rates were ranged between 0.45 and 0. 50 dbl/day in the presence of glucose. They showed no growth in the medium without glucose. The maximum cellular density (2.8 x 10^6 cells/ml) and growth rate (1.30 dbl/day) of T. chui were obtained with 10 mM glucose. For growing this organism, ammonium sulfate was better nitrogen source than sodium nitrate and the maximum growth occurred at a cencentration of 0.6 mM ammonium sulfate in the presence of 10 mM glucose. The addition of glucose was effective to considerably reduce illumination time to obtain the maximum growth.
福山大学の論文